Review



anti-phosphopkc (pan)  (Cell Signaling Technology Inc)


Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90

    Structured Review

    Cell Signaling Technology Inc anti-phosphopkc (pan)
    Anti Phosphopkc (Pan), supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phosphopkc+pan/primary+antibodies/pm40558515-52-20-22
    Average 90 stars, based on 1 article reviews
    anti-phosphopkc (pan) - by Bioz Stars, 2026-10
    90/100 stars

    Images

    Related Articles

    Incubation:

    Article Title: Spinal mediators that may contribute selectively to antinociceptive tolerance but not other effects of morphine as revealed by deletion of GluR5
    Article Snippet: Equal amounts of total protein (50 μg) were subjected to SDS-PAGE using 10% Tris-HCl precast gels (BioRad) and then transferred to PVDF membranes (Millipore, Bedford, MA), which were subsequently blocked in 5% blotting grade milk (BioRad) overnight at 4°C. .. Blots were incubated in primary antibodies generated in rabbit for phosphoP38α/β MAPK Thr180/Tyr182 at 1:250, phosphoERK1/2 MAPK Thr202/Tyr204 at 1:1000, phosphoPKC(pan)γThr514 at 1:1000, P38α/β at 1:500, ERK1/2 at 1:5000 (Cell Signaling Technology, Beverly, MA), PKCγ at 1:20000 (Santa Cruz Biotechnology, Santa Cruz, CA) overnight at 4°C, then incubated either with HRP-conjugated anti-rabbit secondary antibodies at 1:20000 (Pierce, Rockford, IL) for 1 hour at room temperature. .. As a loading control, blots were probed for β-actin using a mouse monoclonal antibody 1:100000 – 1:200000 (Sigma) followed by HRP-conjugated anti-mouse secondary antibodies 1:100000 (Pierce), both for 1 hour at room temperature.

    Article Title: Glibenclamide activates translation in rat pancreatic beta cells through calcium-dependent mTOR, PKA and MEK signalling pathways.
    Article Snippet: .. Samples of 2×105 to 3×105 cells were run on a 12% SDS-polyacrylamide gel before protein transfer on a nitrocellulose membrane and incubation with antibodies against: 4E-BP1 (Santa Cruz, Santa Cruz, CA, USA), total eIF2α, phospho-eIF2α (Ser51), total rpS6, phospho-rpS6 (Ser235/236), total eEF2, phosphoeEF2 (Thr56), phospho-PKB/Akt (Ser473) and phosphoPKC pan (βII Ser660) (all from Cell Signaling Technology), as well as phospho-p42/44 ERK(Thr202/Tyr204) (New England Biolabs, Ipswich, MA, USA) and actin (Santa Cruz). .. Horseradish peroxidase-linked anti-rabbit, antimouse or anti-goat Ig (1:1,000; Amersham, Little Chalfont, UK) were used as second antibodies and peroxidase activities were detected by enhanced chemiluminescence (Amersham).

    Article Title: Alpha1-adrenoceptors down-regulate ClC-2 chloride channels in epithelial cells from the acutely denervated jejunum.
    Article Snippet: Acute sympathetic denervation of the small intestine up-regulates α1-adrenoceptors on villus enterocytes and activation of these α1adrenoceptors inhibits chloride secretion.. We tested whether α1-adrenoceptor-mediated inhibition of chloride secretion was the result of reduced ClC-2 chloride channel expression.. Phorbol myristate acetate (PMA) (a protein kinase C (PKC) activator) had no effect on ClC-2 levels.

    Generated:

    Article Title: Spinal mediators that may contribute selectively to antinociceptive tolerance but not other effects of morphine as revealed by deletion of GluR5
    Article Snippet: Equal amounts of total protein (50 μg) were subjected to SDS-PAGE using 10% Tris-HCl precast gels (BioRad) and then transferred to PVDF membranes (Millipore, Bedford, MA), which were subsequently blocked in 5% blotting grade milk (BioRad) overnight at 4°C. .. Blots were incubated in primary antibodies generated in rabbit for phosphoP38α/β MAPK Thr180/Tyr182 at 1:250, phosphoERK1/2 MAPK Thr202/Tyr204 at 1:1000, phosphoPKC(pan)γThr514 at 1:1000, P38α/β at 1:500, ERK1/2 at 1:5000 (Cell Signaling Technology, Beverly, MA), PKCγ at 1:20000 (Santa Cruz Biotechnology, Santa Cruz, CA) overnight at 4°C, then incubated either with HRP-conjugated anti-rabbit secondary antibodies at 1:20000 (Pierce, Rockford, IL) for 1 hour at room temperature. .. As a loading control, blots were probed for β-actin using a mouse monoclonal antibody 1:100000 – 1:200000 (Sigma) followed by HRP-conjugated anti-mouse secondary antibodies 1:100000 (Pierce), both for 1 hour at room temperature.

    other:

    Article Title: Gp35/50 mucin molecules of Trypanosoma cruzi metacyclic forms that mediate host cell invasion interact with annexin A2.
    Article Snippet: After 1 h incubation of HeLa cells with MT at MOI = 10 (CL strain) or MOI = 20 (G strain), or with EA at MOI = 5, the cells were fixed with Bouin’s solution for 5 min, washed and stained with Giemsa solution (SigmaAldrich/Merck), followed by sequential dehydration with acetone, acetone:xylol, xylol.

    Membrane:

    Article Title: Glibenclamide activates translation in rat pancreatic beta cells through calcium-dependent mTOR, PKA and MEK signalling pathways.
    Article Snippet: .. Samples of 2×105 to 3×105 cells were run on a 12% SDS-polyacrylamide gel before protein transfer on a nitrocellulose membrane and incubation with antibodies against: 4E-BP1 (Santa Cruz, Santa Cruz, CA, USA), total eIF2α, phospho-eIF2α (Ser51), total rpS6, phospho-rpS6 (Ser235/236), total eEF2, phosphoeEF2 (Thr56), phospho-PKB/Akt (Ser473) and phosphoPKC pan (βII Ser660) (all from Cell Signaling Technology), as well as phospho-p42/44 ERK(Thr202/Tyr204) (New England Biolabs, Ipswich, MA, USA) and actin (Santa Cruz). .. Horseradish peroxidase-linked anti-rabbit, antimouse or anti-goat Ig (1:1,000; Amersham, Little Chalfont, UK) were used as second antibodies and peroxidase activities were detected by enhanced chemiluminescence (Amersham).

    Bioprocessing:

    Article Title: TNFalpha activation of PKCdelta, mediated by NFkappaB and ER stress, cross-talks with the insulin signaling cascade.
    Article Snippet: Abbreviations: β-Gal, β-Galactosidase; ER, Endoplas acid; HEK, Human Embryonic Kidney; IκBα-SR, IκBα su IR, Insulin receptor; IRS, Insulin receptor substrate; JNK Extracellular-regulated protein kinase; MOI, Multiplic kinase C; shRNA, Short hairpin RNA; TNFα, Tumor necr ⁎ Corresponding author.. Bassett Research Institute, O NY 13326, United States.. Tel.

    Article Title: TAK1-mediated phosphorylation of PLCE1 represses PIP2 hydrolysis to impede esophageal squamous cancer metastasis
    Article Snippet: Methods Antibodies, inhibitors, and growth factors The following antibodies were used: rabbit polyclonal antiPLCE1 (#PA5100856; Invitrogen, Carlsbad, CA, USA). .. Rabbit monoclonal antibodies against TAK1 (#5206), phosphoTAK1 (Ser412, #9339), PKCα (#2056), phosphoPKC (pan) (gamma Thr514) (#38938), GSK3β (#9315), phosphoGSK3β (Ser9) (#5558), β-Catenin (#8480), phospho-β-Catenin (Ser33/37/Thr41) (#9561), MMP2 (#87809), mouse monoclonal antibodies against Actin (#3700), MycTag (Sepharose Bead Conjugate) (#55464), EpithelialMesenchymal Transition (EMT) antibody sample kit (#9782), phosphorIKK (#2078), IKK (#61294), phosphorJNK (#4668), JNK (#9252), phosphoERK (#4370), ERK (#9102), phosphoP38 MAPK (#9211), P38 MPAK (#9212), and normal rabbit IgG (#2729) were from Cell Signaling Technology (Beverley, MA, USA). .. TAK1 inhibitors 5Z7oxozeaenol (O9890), NG25 (SML1332), and Takinib (SML2216) (#662009) were purchased from SigmaAldrich (St. Louis, MO, USA).



    Similar Products

    90
    Thermo Fisher phosphopkc pan (thr497) polyclonal antibody thermo fisher
    Phosphopkc Pan (Thr497) Polyclonal Antibody Thermo Fisher, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phosphopkc+pan/antibody+++anti+phospho+pkc+pan++thr497+++rabbit+polyclonal+/pm31891789-95-13-18
    Average 90 stars, based on 1 article reviews
    phosphopkc pan (thr497) polyclonal antibody thermo fisher - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    90
    Cell Signaling Technology Inc anti-phosphopkc (pan)
    Anti Phosphopkc (Pan), supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phosphopkc+pan/primary+antibodies/pm40558515-52-20-22
    Average 90 stars, based on 1 article reviews
    anti-phosphopkc (pan) - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    93
    Cell Signaling Technology Inc phosphopkc pan
    Phosphopkc Pan, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phosphopkc+pan/Phospho-PKC+(pan)+(gamma+Thr514)+Rabbit+mAb/10__7554_slash_elife__97373__3-406-11-71
    Average 93 stars, based on 1 article reviews
    phosphopkc pan - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    90
    Affinity Biosciences phosphopkc-pan
    Phosphopkc Pan, supplied by Affinity Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phosphopkc+pan/phosphopkc+pan/pm33047600-30-1-8
    Average 90 stars, based on 1 article reviews
    phosphopkc-pan - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    90
    Cell Signaling Technology Inc phosphopkc (pan) primary antibody #9371
    Phosphopkc (Pan) Primary Antibody #9371, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phosphopkc+pan/phosphopkc++pan++primary+antibody++9371/pm32446889-50-0-8
    Average 90 stars, based on 1 article reviews
    phosphopkc (pan) primary antibody #9371 - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    96
    Cell Signaling Technology Inc pan phosphopkc ser660
    Pan Phosphopkc Ser660, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phosphopkc+pan/Phospho-PKC+(pan)+(betaII+Ser660)+Antibody/pm29719042-67-37-2
    Average 96 stars, based on 1 article reviews
    pan phosphopkc ser660 - by Bioz Stars, 2026-10
    96/100 stars
      Buy from Supplier

    90
    Cell Signaling Technology Inc anti phosphopkc (pan) (βii ser660)
    Anti Phosphopkc (Pan) (βii Ser660), supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phosphopkc+pan/primary+antibodies/pm28583844-60-6-14
    Average 90 stars, based on 1 article reviews
    anti phosphopkc (pan) (βii ser660) - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    96
    Cell Signaling Technology Inc phosphopkc
    Fig. 6 PKC signals downstream upon TsSP-MR interaction. Quantification of flow cytometry analysis of the expression levels of (a) CCR2, (b) LFA-1 avidity and (c) LFA-1 affinity on human monocytes. These cells were cultured in the presence or absence of TsSP (40 μg/ml, 16 h), the PKC inhibitor Bisindolylmaleimide I (GF109203, 2 μM) and blocking antibodies for the MR or its isotype control (both 10 μg/ml). (b) and (c) are expressed as the percentage of avidity/affinity compared to total LFA-1. Cell supernatants were used to determine the levels of (d) TNF-α and (e) IL-10 secretion by enzyme-linked immunosorbent assay analysis. f PKC activation was determined by Western Blotting using <t>phosphoPKC</t> antibodies compared to GAPDH expression and images were subsequently quantified using ImageJ. Experiments were performed in triplicate using cells derived from 4 different human donors and the results are presented as the mean (a-c); the mean percentage (d, e) or the mean PKC phosphorylation +/−SEM. *p < 0.05, **p < 0.01, ***p < 0.001 as determined by ANOVA and Students t test
    Phosphopkc, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phosphopkc+pan/Phospho-PKC+(pan)+(betaII+Ser660)+Antibody/pm26205402-74-12-13
    Average 96 stars, based on 1 article reviews
    phosphopkc - by Bioz Stars, 2026-10
    96/100 stars
      Buy from Supplier

    Image Search Results


    Fig. 6 PKC signals downstream upon TsSP-MR interaction. Quantification of flow cytometry analysis of the expression levels of (a) CCR2, (b) LFA-1 avidity and (c) LFA-1 affinity on human monocytes. These cells were cultured in the presence or absence of TsSP (40 μg/ml, 16 h), the PKC inhibitor Bisindolylmaleimide I (GF109203, 2 μM) and blocking antibodies for the MR or its isotype control (both 10 μg/ml). (b) and (c) are expressed as the percentage of avidity/affinity compared to total LFA-1. Cell supernatants were used to determine the levels of (d) TNF-α and (e) IL-10 secretion by enzyme-linked immunosorbent assay analysis. f PKC activation was determined by Western Blotting using phosphoPKC antibodies compared to GAPDH expression and images were subsequently quantified using ImageJ. Experiments were performed in triplicate using cells derived from 4 different human donors and the results are presented as the mean (a-c); the mean percentage (d, e) or the mean PKC phosphorylation +/−SEM. *p < 0.05, **p < 0.01, ***p < 0.001 as determined by ANOVA and Students t test

    Journal: Acta neuropathologica communications

    Article Title: Trichuris suis induces human non-classical patrolling monocytes via the mannose receptor and PKC: implications for multiple sclerosis.

    doi: 10.1186/s40478-015-0223-1

    Figure Lengend Snippet: Fig. 6 PKC signals downstream upon TsSP-MR interaction. Quantification of flow cytometry analysis of the expression levels of (a) CCR2, (b) LFA-1 avidity and (c) LFA-1 affinity on human monocytes. These cells were cultured in the presence or absence of TsSP (40 μg/ml, 16 h), the PKC inhibitor Bisindolylmaleimide I (GF109203, 2 μM) and blocking antibodies for the MR or its isotype control (both 10 μg/ml). (b) and (c) are expressed as the percentage of avidity/affinity compared to total LFA-1. Cell supernatants were used to determine the levels of (d) TNF-α and (e) IL-10 secretion by enzyme-linked immunosorbent assay analysis. f PKC activation was determined by Western Blotting using phosphoPKC antibodies compared to GAPDH expression and images were subsequently quantified using ImageJ. Experiments were performed in triplicate using cells derived from 4 different human donors and the results are presented as the mean (a-c); the mean percentage (d, e) or the mean PKC phosphorylation +/−SEM. *p < 0.05, **p < 0.01, ***p < 0.001 as determined by ANOVA and Students t test

    Article Snippet: The following primary antibodies (in TSM/0,05 % Tween/5 % BSA) were used: PhosphoPKC (Cell signaling, Beverly, MA, USA, #9371S) and GAPDH (Santa Cruz Biotechnology, Heidelberg, Germany, #sc-32233) as a loading control.

    Techniques: Flow Cytometry, Expressing, Cell Culture, Blocking Assay, Control, Enzyme-linked Immunosorbent Assay, Activation Assay, Western Blot, Derivative Assay, Phospho-proteomics